Analysis and Comparison of Major Immune Cell Populations in Peripheral Blood of Naïve NHP
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Analysis and Comparison of Major Immune Cell Populations in Peripheral Blood of Naïve NHP presented by Andrea Wong, Scientist, Flow Cytometry
Authors: M. Patel, A. S. Wong, and T. Rogers. Altasciences, Seattle, WA.
Changes in major immune cell populations in the peripheral blood of cynomolgus monkeys, as determined by flow cytometry, are routinely used as an immunological endpoint in toxicology studies. However, it is difficult, especially for studies involving small animal groups, to determine a “normal” value that can be used as a guideline. To determine what constitutes a “normal” value for various immune cell populations in the peripheral blood, we analyzed large cohorts of naïve adolescents (total: n=168, males: n=84, females: n=84) and infant (n=33) cynomolgus monkeys. We determined relative percentage and absolute counts of immune cell populations, such as CD3+ total T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, CD3-CD20+ B cells, CD3-CD16+ NK cells, and CD3-CD14+ monocytes. The number of CD3+ total T cells was 3.06±1.25, 3.24±1.35, 2.88±1.12, and 4.97±1.55 x 106 per mL for total adolescent, male, female, and infant cynomolgus monkeys, respectively. The number of B cells (CD3-CD20+) were 1.34±0.75, 1.45±0.90, 1.23±0.56, 1.85±1.01x 106 per mL and the number of NK cells (CD3-CD16+) were 7.59±4.24, 8.56±4.44, 6.63±3.81, 4.48±1.94 x 105 per mL for total adolescent, male, female, and infant cynomolgus monkeys, respectively. The numbers for monocyte counts (CD3-CD14+) were 2.48±1.09, 2.46±1.22, 2.50±0.94, and 4.44±2.08 x 105 per mL for total adolescent, male, female, and infant cynomolgus monkeys, respectively. Our results also indicated statistically significant differences in absolute counts of various cell populations based on the age and sex of cynomolgus monkeys. For example, infants had significantly higher levels of total lymphocytes, CD3-CD14+ monocytes, CD3+ total T cells, CD4+ T cells, and CD3-CD20+ B cells compared to adolescent monkeys (p<0.005, Mann Whitney test). The absolute counts of CD3-CD16+ NK cells, were significantly lower in infants compared to adolescent monkeys (p<0.0001, Mann Whitney test). In addition, we also observed sex-dependent differences where females had significantly lower counts of CD3+CD4+ T cells and CD3-CD16+ NK cells compared to males ((p<0.001, Mann Whitney test). Overall, our data helps understand the various immune cell populations present in peripheral blood cynomolgus monkeys. The reported values can also serve as a guideline for improved understanding of the various toxicology related changes introduced by therapeutic treatments during drug development.
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