Procedure Development for Repeated Infusion in Juvenile Non-Human Primates
Presented at AALAS National Meeting 2019; October 13-17, 2019; Denver, Colorado
The method for infusion restraint included the use of a procedure cage where all limbs were secured with limited movement. This method would be highly stressful for animals under 1 year of age. For previous infusion dosing of pre-weaned infants at 3 months, the “snuggle wrap” was developed to allow young animals to remain immobile while still feeling comforted by a hugging-like sensation. Stress behaviors were noticeably reduced when using the snuggle wrap compared to manually holding as a restraint method. The snuggle wraps were designed for ease of dosing vein accessibility while the remaining limbs are secure and comfortable. Knowing social pairs needed to remain in proximity, the “snuggle board” was then developed. Animals were implanted with RFID chips, and the wrapped juveniles were labeled with temporary ID cards to allow for easy identification once snuggled and placed on the board. These boards hold 4 snuggled animals closely together which keeps social pairs in visual and auditory contact during dose administration. Following catheter placement, dosing limbs were secured with Velcro straps for dose duration. Animals were visually monitored, provided treats, juice, light projections, and chewing toys throughout dose duration by trained technicians. The snuggle board restraints were used for ~13 weeks, until the animals were over 1 year of age and large enough for the procedure cage.
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