PBMC Isolation
PBMC Isolation and Clinical Flow Cytometry Analysis for Clinical Trials
Do you need a proven partner for Peripheral Blood Mononuclear Cell (PBMC) isolation and analysis for your early-phase clinical trial?
At Altasciences, our advanced capabilities for the isolation and processing of PBMCs utilize state-of-the-art methodologies and equipment. Our validated PBMC isolation workflows minimize variability and maximize immune marker fidelity, helping you detect drug-related immunological effects with confidence. Our rigorous harvesting, processing, and analysis techniques maximize cell recovery and maintain high viability, even before freezing. Our expertise in this important process helps you:
- Evaluate immune responses and analyze the influence of the investigational product on the immune system at various stages of the response.
- Perform long-term experimentation.
- You can continue conducting experiments on collected cells under different conditions, such as inflammatory environments, the presence of specific biomarkers, and analyzing vaccine response
We ensure fast and reliable PBMC isolation results across patient populations.
Consult our Flow Cytometry and PBMC Fact Sheet
Specialized Expertise in Early-Phase Clinical PBMC Isolation and Processing
Our combination of leading-edge technology, scalable methodologies, and a highly experienced team ensures that your PBMC isolation and processing needs are met with efficiency and high quality. We support PBMC isolation and downstream immune cell profiling using advanced flow cytometry platforms.
By leveraging our capabilities in both whole blood and cryopreserved PBMC isolation and analysis, your clinical trial samples will be handled with care and precision, providing you with the reliable data you need to drive your research forward. Partner with Altasciences in advancing immune response assessments and therapeutic development.
With a focus on efficiency, scalability, and precision, we offer tailored solutions that meet the specific needs of your clinical trials, ensuring high-quality results at every stage.
Multiple Methodologies to Deliver Reliable PBMC Isolation Data, Quickly
The multiple PBMC isolation methods we use ensure optimal results based on the unique requirements of each study.
Methodologies
- Cell Preparation Tube (CPT):
- Ensures highly reproducible results across staff members, reducing variability.
- Minimizes the time from blood draw to cryopreservation, preserving cell viability and functionality.
- Cost-effective due to decreased labor requirements, allowing for more frequent collections in studies.
- Ficoll-Paque:
- Used for isolating human lymphocytes for in vitro studies
- Offers greater scalability for processing different sample sizes, making it ideal for larger studies requiring flexibility.
- LymphoPrep Tubes:
- Provide the same scalability for varying sample sizes.
- Help technicians avoid red blood cell (RBC) contamination more effectively, resulting in cleaner samples and improved data integrity.
Advanced PBMC Processing and High-Yield Isolation Methods
Leading-Edge Equipment
Altasciences' PBMC isolation labs are equipped with advanced tools to support high-throughput processing while maintaining the highest standards of quality. Whether your needs are for rapid turnaround during a dose escalation study, or stand-alone services for an ongoing study requiring local site assistance, we can cover your needs. We can process over 200 samples per day using our state-of-the-art cell counters.
Skilled Team and Processing Capabilities
Our team of highly trained and certified professionals has extensive experience in PBMC isolation.
- Expert Training: Trained by specialists at the Stanford School of Medicine, ensuring adherence to the highest quality standards in sample processing.
- Ph.D. Oversight: Every project is overseen by experienced Ph.D.-level scientists, guaranteeing scientific rigor and precision.
- Scalable Processing Capacity: Capable of processing 60 mL of blood per patient per day for up to 33 patients in a single day, enabling efficient support for studies of any size and complexity.
- Cryopreserved PBMC Expertise: Proven excellence in using cryopreserved PBMCs as a matrix for flow cytometry. We ensure cell viability and protein stability to maximize data quality, particularly for rare immune cell subsets.
- Strategic Proximity for Rapid Analysis: Clinics and laboratories in the U.S. and Canada are strategically located to ensure seamless sample transitions and rapid analysis turnaround.
Related Resources
Fact Sheets
Webpage
FAQS
What are PBMCs?
PBMCs are a diverse population of cells essential for understanding immune response, developing regenerative therapies, and advancing revolutionary cancer treatments. PBMCs also provide valuable insights into autoimmune diseases and serve as indicators of gene expression and disease biomarkers. PBMCs are an essential cell population with applications in numerous fields of clinical research.
How are PBMCs analyzed?
Quality bioanalytical support is essential for accurate and timely assessment of primary, secondary, and exploratory endpoints involving immunological markers and cellular responses, such as those provided by PBMC isolation. Our clinical facilities are integrated with state-of-the-art flow cytometry laboratories, equipped with advanced platforms and staffed by an in-house team of experts proficient in regulated cellular analysis. We specialize in developing and validating assays tailored to your specific program requirements, ensuring efficient, timely, high-quality analysis of PBMCs for your clinical trials.
Does Altasciences work with fresh or cryopreserved blood samples?
Altasciences has experience in performing PBMC isolation and analysis in both fresh whole and cryopreserved blood samples. For samples susceptible to the cryopreservation process, such as RO assessment, we can offer custom assay design; while for samples with a narrow window of stability, we design panels in whole blood and have an array of strategies to extend sample stability.