Breaking Ground: A Novel Method for Whole Blood Linearity Assessments

Achieving precise and relevant dilutions while mitigating variations in sample preparation poses a significant challenge.

In this study, we assessed:

  • the impact of four different linearity sample preparation methods, including fixed FMX, fixed unconjugated, fresh FMX, and fresh unconjugated.
  • the accuracy of quantifying low-abundance cell populations (T regulatory cells and CD14-CD16+ monocytes) using a lyse/no wash method for whole blood staining.

Our results demonstrate that one of the novel sample preparation methods showed superior performance compared to the industry-standard approach, leading to a marked improvement in linearity assessment quality.

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